NC - Nucleo-Capsid protein
HIV NC binds to the HIV packaging signal on viral RNA and is sufficient for incorporation of RNA into virions.
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[animated gif: 320x240 ]
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Isoforms:
Cleavage site:
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Localization:
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Function:
- structural protein
- incorporates viral RNA into virion
- specific recognition of HIV packaging signal of viral RNA (Ref. #4)
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Additional
Information:
- the HIV packaging signal consists of 4 stem-loop structures near the 5'-end of viral RNA
- the HIV packaging signal is sufficient to mediate the incorporation of heterologous RNA into virions (Ref. #5)
- NC contains two zinc-finger motifs, which interact with the 4 stem-loop structures of the HIV packaging signal (Ref. #6)
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Genomic Location: [TOP]
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Reference
Sequences:
HIV-1
(HXB2):
10 20 30 40 50
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MQRGNFRNQR KIVKCFNCGK EGHTARNCRA PRKKGCWKCG KEGHQMKDCT ERQAN
[download in fasta format]
Length:
55 amino acids
Molecular Weight: 6425 Da
Theoretical pI: 10.08
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Protein Domains/Folds/Motifs: [TOP]
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InterPro signature for Retroviral NucleoCapsid protein Gag - IPR000721
Secondary Structure prediction:
Antigenic Sites - EMBOSS:
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N-glycosylation:
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N-myristoylation:
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Amidation:
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Protein kinase C:
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Casein kinase II:
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Tyrosine kinase:
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cAMP / cGMP kinase:
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Cell attachment motif:
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Asp Protease motif:
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Asp Prot Retro motif:
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Cysteine-rich Region:
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Tryptophan-rich Region:
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Zinc-finger CCHC motif:
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Leucine Zipper motif:
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Protein-Protein Interactions: [TOP]
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Primary and Secondary Database Entries: [TOP]
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Identifiers:
PDB/MMDB: Search for HIV-1 & Nucleocapsid
SwissProt: P04591 (HIV-1 HXB2 GAG)
EMBL: K03455; AAB50258.1 [EMBL/GenBank/DDBJ]
PIR: UNKNOWN
HIV: K03455; UNKNOWN$HXB2
InterPro: IPR001878
Pfam: PF00607 / PF00098
Prints: none
ProDom:
PD000129 (1 - 36) /
PD506618 (3 - 37) /
PD456461 (11 - 55) /
PD594906 (14 - 51)
PD375076 (15 - 49) /
PD107922 (15 -55) /
PD019096 (15 -53) /
PD406622 (37 -55)
SCOP: SSF57756 Retroviral zinc finger-like domains
BLOCKS: P04591
Prosite: P04591
ProtoNet: P04591
ProtoMap: P04591
PRESAGE: P04591
Database of Interacting Proteins: P04591
ModBase: P04591
Swiss-2DPAGE: 2D gel
- BioAfrica Tools:
- - Gag Protein Data Mining Tool provides real-time analysis of HIV-1 Gag isolates
- - HIV Structure BLAST searches for similar HIV sequences that have known structures
- - HIV Proteomics Resource contains protein sequence and structure analysis tools
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Reviews and References: [TOP]
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- 1 - HIV Sequence Compendium 2000
- Kuiken CL, Foley B, Hahn B, Korber B, Marx PA, McCutchan F, Mellors JW, Mullins JI, Sodroski J, Wolinksy S.
- Theoretical Biol. & Biophys. Group, Los Alamos Nat Lab, LA-UR 01-3860 [Read it online: Compendium]
- 2 - Retroviruses
- Coffin JM, Hughes SH, Varmus HE.
- CD-ROM ed. (2002) Cold Spring Harbor Laboratory Press [Read it online: NCBI Bookshelf]
- 3 - Molecular Characteristics of HIV-1 Subtype C Viruses from KwaZulu-Natal, South Africa:
- Implications for Vaccine and Antiretroviral Control Strategies.
- Gordon M, De Oliveira T, Bishop K, Coovadia HM, Madurai L, Engelbrecht S, Janse van Rensburg E, Mosam A, Smith A, Cassol S.
- Journal of Virology 77(4): 2587-2599 (2003) [pubmed: 12551997]
- 4 - The human immunodeficiency virus type 1 packaging signal and major splice donor region
- have a conserved stable secondary structure.
- Harrison GP, Lever AM.
- J Virol 66: 4144-4153 (1992) [pubmed: 1602537]
- 5 - Gene transfer into human lymphocytes by a defective human immunodeficiency virus type 1 vector.
- Poznansky M, Lever A, Bergeron L.
- J Virol 65: 532-536 (1991) [pubmed: 1985215]
- 6 - Interactions between HIV-1 nucleocapsid protein and viral DNA may have important functions
- in the viral life cycle.
- Lapadat-Tapolsky M, De Rocquigny H, Van Gent D.
- Nucleic Acids Res 21: 831-839 (1993) [pubmed: 8383840]
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