P6 GAG protein

HIV p6 mediates interactions between p55 and Vpr, leading to the incorporation of Vpr into assembling virions.



STRUCTURE NOT AVAILABLE

Key web links:
ViralZone: HIV-1, HIV replication cycle, HIV resource
PDB: not available
UniProt: P04591 (HIV-1 HXB2 GAG)
Chime Tutorial: not available
HIV-1/Human Protein Interaction DB: HIV-1 Gag
Los Alamos HIV structure DB: not available
EMBL: K03455 [EMBL/GenBank/DDBJ]


Isoforms:

  • p6 (52 amino acids)

Cleavage sites:


Function:

  • As part of the Gag polyprotein, p6 facilitates virus ESCRT-dependent budding through two distinct late assembly domains (L-domains) and allows the incorporation of Vpr into virus
    particles .
  • As part of the Gag polyprotein, mediates interactions between p55 and Vpr .

Localization:

  • Virion
  • Cell cytoplasm

Additional Information:

  • Late timing of expression.
  • Contains a late domain, which is necessary for the efficient release of budding virions from an infected cell.

Gag-p6 Function & Host-Virus Protein Interactions:[TOP]




Interactions highlighted in the image:


Potential interactions (not in the image):


  • P6 interacts with Gag (p55) polyprotein and Vpr (when phosphorylated).
  • P6 interacts with proteins involved in the human multivesicular bodies (MVB) biogenesis pathway facilitating budding of virions.


Genomic Location & Protein Sequence: [TOP]

HIV-1 (HXB2):

          10         20         30         40         50 
| | | | |
LQSRPEPTAP PEESFRSGVE TTTPPQKQEP IDKELYPLTS LRSLFGNDPS SQ

[download in fasta format]

Length: 52 amino acids
Molecular Weight: 5784 Da
Theoretical pI: 4.60
Position relative to Gag protein: 449 - 500


Protein Domains/Folds/Motifs: [TOP]

Secondary Structure prediction:

Antigenic Sites - EMBOSS:

Predicted Motifs: Printer-friendly version

N-glycosylation:
N-myristoylation:
Amidation:
Protein kinase C:
Casein kinase II:
Tyrosine kinase:
cAMP / cGMP kinase:
Cell attachment motif:
Asp Protease motif:
Asp Prot Retro motif:
Cysteine-rich Region:
Tryptophan-rich Region:
Zinc-finger CCHC motif:
Leucine Zipper motif:

Primary and Secondary Database Entries: [TOP]

Identifiers:



ViralZone: HIV-1, HIV replication cycle, HIV resource
PDB/MMDB: Search for HIV & p6

UniProt: P04591 (HIV-1 HXB2 GAG)
EMBL: K03455; AAB50258.1 [EMBL/GenBank/DDBJ]

InterPro: none
Pfam: UNKNOWN [see Gag Pfam (P04591)]
Prints: none
SCOP: none
BLOCKS: P04591
Prosite: P04591
ProtoNet: P04591
Database of Interacting Proteins: P04591
ModBase: P04591
HIV-1/Human Protein Interaction DB: HIV-1 Gag
HIV-1 Sequence Database Los Alamos HIV Sequence Database

PDB:




Reviews and References: [TOP]

Cite the resource by citing the following paper:
Doherty R et al. BioAfrica's HIV-1 Proteomics Resource: Combining protein data with bioinformatics tools. Retrovirology (2005), 9;2(1):18.

1 - HIV Sequence Compendium 2000
Kuiken CL, Foley B, Hahn B, Korber B, Marx PA, McCutchan F, Mellors JW, Mullins JI, Sodroski J, Wolinksy S.
Theoretical Biol. & Biophys. Group, Los Alamos Nat Lab, LA-UR 01-3860 [Read it online: Compendium]
2 - Retroviruses
Coffin JM, Hughes SH, Varmus HE.
CD-ROM ed. (2002) Cold Spring Harbor Laboratory Press [Read it online: NCBI Bookshelf]
3 - Molecular Characteristics of HIV-1 Subtype C Viruses from KwaZulu-Natal, South Africa:
Implications for Vaccine and Antiretroviral Control Strategies.
Gordon M, De Oliveira T, Bishop K, Coovadia HM, Madurai L, Engelbrecht S, Janse van Rensburg E, Mosam A, Smith A, Cassol S.
Journal of Virology 77(4): 2587-2599 (2003) [pubmed: 12551997]
4 - Incorporation of Vpr into human immunodeficiency virus type 1 virions: Requirement for
the p6 region of gag and mutational analysis.
Paxton W, Connor RI, Landau NR.
J Virol 67: 7229-7237 (1993) [pubmed: 8230445]
5 - The protein network of HIV budding
von Schwedler UK, Stuchell M, Muller B, Ward DM, Chung HY, Morita E, Wang HE, Davis T, He GP, Cimbora DM, Scott A, Krausslich HG, Kaplan J, Morham SG, Sundquist WI.
Cell 114(6): 701-713 (2003) [pubmed: 14505570]
6 - Wrapping up the bad news: HIV assembly and release.
Meng B, Lever AM.
Retrovirology 10:5 (2013) [pubmed: 23305486]
7 - The phosphorylation of HIV-1 Gag by atypical protein kinase C facilitates viral infectivity by promoting Vpr incorporation into virions.
Kudoh A, Takahama S, Sawasaki T, Ode H, Yokoyama M, Okayama A, Ishikawa A, Miyakawa K, Matsunaga S, Kimura H, Sugiura W, Sato H, Hirano H, Ohno S, Yamamoto N, Ryo A.
Retrovirology 11:9 (2014) [pubmed: 24447338]
8 - Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding.
Garrus JE, von Schwedler UK, Pornillos OW, Morham SG, Zavitz KH, Wang HE, Wettstein DA, Stray KM, Côté M, Rich RL, Myszka DG, Sundquist WI.
Cell 107(1):55-65 (2001) [pubmed: 11595185]
9 - AIP1/ALIX is a binding partner for HIV-1 p6 and EIAV p9 functioning in virus budding.
Strack B, Calistri A, Craig S, Popova E, Göttlinger HG.
Cell 114(6):689-99 (2003) [pubmed: 14505569]



Thanks to ViralZone at the Swiss Institute of Bioinformatics (SIB) for allowing the use of their images on bioafrica.net.
Page last updated by Megan Druce, Paula Sommer and Tulio de Oliveira (UKZN/Africa Centre) & Philippe Le Mercier, Chantal Hulo and Patrick Masson (SIB/ViralZone).